Pengaruh Ekstrak Rasberi (Rubus occidentalis) terhadap Kadar Asam Urat Tikus Wistar Model Sindrom Metabolik

Milana Phangadi; Jarot Subandono; Sarsono -

doi:10.20961/plexus.v1i3.48

Authors

Milana Phangadi Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta, Indonesia
Jarot Subandono Departemen Biokimia, Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta, Indonesia
Sarsono - Departemen Biokimia, Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta, Indonesia

DOI:

https://doi.org/10.20961/plexus.v1i3.48

Keywords:

sindrom metabolik, rasberi, asam urat, tikus wiztar

Abstract

Pendahuluan: Sindrom metabolik yang mencakup hiperinsulinemia, hiperkolestrolemia, dislipidemia, hipertensi, dan juga obesitas dapat memengaruhi kadar asam urat dalam tubuh seseorang. Hiperinsulinemia akan menyebabkan gangguan metabolisme asam urat yang menyebabkan kondisi hiperurisemia. Rasberi merupakan buah yang kaya antosianin yang merupakan flavonoid natural yang menunjukan efektivitas dalam pengobatan sindrom metabolik namun efektivitasnya terhadap kadar asam urat masih kurang banyak diteliti. Penelitian ini bertujuan untuk membuktikan efektivitas rasberi dalam menurunkan kadar asam urat serta melihat pengaruh perbedaan dosis ekstrak rasberi terhadap kadar asam urat pada tikus wistar sindrom metabolik.

Metode: Penelitian ini merupakan penelitian eksperimental laboratorik dengan sampel 30 tikus Wistar dalam 5 kelompok (kontrol dan perlakuan). Tikus diinduksi diet HFFD selama 28 hari lalu diinjeksi STZ-NA dan diukur kadar asam uratnya (pretest) bersama dengan parameter lain (berat badan, glukosa, HDL, dan kolesterol total) untuk memastikan sampel memenuhi kriteria sindrom metabolik. Selanjutnya kelompok perlakuan diberi ekstrak rasberi (Rubus occidentalis) dengan dosis 125 mg/kgBB, 250 mg/kgBB, dan 375 mg/kgBB selama 21 hari lalu diukur kadar asam uratnya (posttest). Kadar asam urat didapat melalui serum darah vena retroorbital yang dianalisis dengan kit DiaSys. Kadar asam urat pretest dan posttest kelompok kontrol dan perlakuan dianalisis menggunakan uji T berpasangan dan One Way ANOVA.

Hasil: Kadar asam urat posttest menurun sebesar 6,39 mg/dL, 7,58 mg/dL, dan 8,74 mg/dL pada kelompok perlakuan. Terdapat perbedaan signifikan (p<0,05) antara rerata kadar asam urat pretest dan posttest serta rerata antar kelompok.

Kesimpulan: Rasberi (Rubus occidentalis) dapat menurunkan kadar asam urat dalam darah dan efeknya semakin besar sebanding dengan jumlah dosis yang diberikan.

Kata Kunci: Sindrom Metabolik, Rasberi (Rubus occidentalis), Asam Urat, Tikus Wiztar

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