Pengaruh Ekstrak Etanolik Daun Kelor (Moringa oleifera, Lam) Terhadap Nilai Ekspresi TNF-α Testis Tikus Wistar (Rattus norvegicus) Model Sindrom Metabolik
DOI:
https://doi.org/10.20961/plexus.v1i4.46Abstract
Latar Belakang: Peningkatan angka penderita sindrom metabolik secara global dianggap mengkhawatirkan karena mengakibatkan komplikasi masalah kesehatan pada berbagai organ termasuk organ reproduksi pria. Infertilitas pada pria yang disebabkan oleh inflamasi kronik dan kematian sel yang terjadi pada sindrom metabolik erat kaitannya dengan peran TNF-α sebagai mediator inflamasi. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanolik daun kelor terhadap ekspresi TNF-α pada jaringan testis tikus Wistar model sindrom metabolik.
Metode: Penelitian bersifat ekperimental laboratorik dengan rancangan penelitian posttest only control group design. Subjek tikus Wistar dibagi menjadi 5 kelompok dengan masing-masing kelompok berisi 6 tikus, KI sebagai kontrol normal, KII sebagai kelompok induksi sindrom metabolik, dan KIII, KIV, KV sebagai kelompok sindrom metabolik yang diberi ekstrak etanolik daun kelor dengan dosis secara berurutan 150 mg/kgBB, 250 mg/kgBB, dan 350 mg/kgBB. Induksi sindrom metabolik menggunakan pakan tinggi lemak dan STZ-NA. Perhitungan ekspresi TNF-α menggunakan metode semikuantitatif IDS. Analisis ekspresi TNF-α menggunakan uji One-Way ANOVA yang dilanjutkan post hoc LSD dan uji regresi linear.
Hasil: Uji One-Way ANOVA ekspresi TNF-α pada jaringan testis menunjukkan p=0,001 (p<0,05), maka terdapat perbedaan ekspresi TNF-α yang signifikan pada kelima kelompok. Perbedaan bermakna terdapat pada KI terhadap KII dan KII terhadap KIV dan KV. Uji regresi linear menunjukkan pengaruh dosis ekstrak etanolik daun kelor memberikan arah negatif terhadap ekspresi TNF-α.
Simpulan: Pemberian ekstrak etanolik daun kelor (Moringa oleifera, Lam) dengan dosis 150 mg/kgBB, 250 mg/kgBB, dan 350 mg/kgBB dapat menurunkan ekspresi TNF-α.
Kata Kunci: TNF-α, Ekstrak Etanolik Daun Kelor, Sindrom Metabolik, Testis, Spermatogenesis
Keywords:
TNF-α, Ekstrak Etanolik Daun Kelor, Sindrom Metabolik, Testis, SpermatogenesisReferences
Asadi, N., Bahmani, M., Kheradmand, A., & Rafieian-Kopaei, M. (2017). The impact of oxidative stress on testicular function and the role of antioxidants in improving it: A review. Journal of Clinical and Diagnostic Research, 11(5), 1–5. https://doi.org/10.7860/JCDR/2017/23927.9886
Bhattacharya, A., Tiwari, P., Sahu, P. K., & Kumar, S. (2018). A Review of the Phytochemical and Pharmacological Characteristics of Moringa oleifera. Asian Journal of Pharmaceutical and Clinical Research, 10(4), 181–191. https://doi.org/10.4103/jpbs.JPBS_126_18
Bitew, Z. W., Alemu, A., Ayele, E. G., Tenaw, Z., Alebel, A., & Worku, T. (2020). Metabolic syndrome among children and adolescents in low and middle income countries: a systematic review and meta-analysis. Diabetology and Metabolic Syndrome, 12(1). https://doi.org/10.1186/s13098-020-00601-8
Dai, X., Zhou, L. Y., Xu, T. T., Wang, Q. Y., Luo, B., Li, Y. Y., Gu, C., Li, S. P., Wang, A. Q., Wei, W. H., & Yang, S. M. (2020). Reproductive responses of the male brandt’s vole, lasiopodomys brandtii (Rodentia: Cricetidae) to tannic acid. Zoologia, 37, 1–11. https://doi.org/10.3897/zoologia.37.e52232
de Moura e Dias, M., dos Reis, S. A., da Conceição, L. L., Sediyama, C. M. N. de O., Pereira, S. S., de Oliveira, L. L., Gouveia Peluzio, M. do C., Martinez, J. A., & Milagro, F. I. (2021). Diet-induced obesity in animal models: points to consider and influence on metabolic markers. Diabetology and Metabolic Syndrome, 13(1). https://doi.org/10.1186/s13098-021-00647-2
Dewi, R. S. (2019). Persepsi Masyarakat Mengenai Obat Tradisional di Kelurahan Simpang Baru Kecamatan Tampan Kota Pekanbaru. Jurnal Penelitian Farmasi Indonesia, 8(2), 75–79. https://doi.org/10.51887/jpfi.v8i2.782
Gopalakrishnan, L., Doriya, K., & Kumar, D. S. (2016). Moringa oleifera: A review on nutritive importance and its medicinal application. Food Science and Human Wellness, 5(2), 49–56. https://doi.org/10.1016/j.fshw.2016.04.001
Hemalatha, S., & Hari, R. (2015). Fertility enhancing effect of saponin rich butanol extracts of tribulus terrestris fruits in male albino rats. International Journal of Pharmaceutical and Clinical Research, 7(1), 36–43.
Irfan, H. M., Khan, N. A. K., & Asmawi, M. Z. (2020). Moringa oleifera Lam. leaf extracts reverse metabolic syndrome in Sprague Dawley rats fed high-fructose high fat diet for 60-days. Archives of Physiology and Biochemistry, 0(0), 1–7. https://doi.org/10.1080/13813455.2020.1762661
Jang, K. J., Ki Kim, H., Han, M. H., Oh, Y. N., Yoon, H. M., Chung, Y. H., Kim, G. Y., Hwang, H. J., Kim, B. W., & Choi, Y. H. (2013). CytoAnti-inflammatory effects of saponins derived from the roots of Platycodon grandiflorus in lipopolysaccharide-stimulated BV2 microglial cells. International Journal of Molecular Medicine, 31(6), 1357–1366. https://doi.org/10.3892/ijmm.2013.1330
Kalliolias, G. D., Ivashkiv, L. B., & Program, T. D. (2016). TNF biology, pathogenic mechanisms and emerging therapeutic strategies. 12(1), 49–62. https://doi.org/10.1038/nrrheum.2015.169.TNF
Kumar, S., Agrawal, D., Sharma, K., & Swain, T. (2015). Association of Male Infertility to Metabolic Syndrome and Other Related Disorders. Journal of Integrative Nephrology and Andrology, 2(4), 107. https://doi.org/10.4103/2225-1243.168524
Leisegang, K., Henkel, R., & Agarwal, A. (2019). Obesity and metabolic syndrome associated with systemic inflammation and the impact on the male reproductive system. American Journal of Reproductive Immunology, 82(5), 1–14. https://doi.org/10.1111/aji.13178
Luetragoon, T., Sranujit, R. P., Noysang, C., Thongsri, Y., Potup, P., Suphrom, N., Nuengchamnong, N., & Usuwanthim, K. (2020). Bioactive compounds in moringa oleifera Lam. Leaves inhibit the pro-inflammatory mediators in lipopolysaccharide-induced human monocyte-derived macrophages. Molecules, 25(1), 1–16. https://doi.org/10.3390/molecules25010191
Marrelli, M., Conforti, F., Araniti, F., & Statti, G. A. (2016). Effects of saponins on lipid metabolism: A review of potential health benefits in the treatment of obesity. Molecules, 21(10), 1–20. https://doi.org/10.3390/molecules21101404
Mbikay, M. (2012). Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: A review. Frontiers in Pharmacology, 3 MAR(March), 1–12. https://doi.org/10.3389/fphar.2012.00024
Nigdelioglu Dolanbay, S., Kocanci, F. G., & Aslim, B. (2021). Neuroprotective effects of allocryptopine-rich alkaloid extracts against oxidative stress-induced neuronal damage. Biomedicine and Pharmacotherapy, 140(April), 111690. https://doi.org/10.1016/j.biopha.2021.111690
Pratiwi, R., Nantasenamat, C., Ruankham, W., Suwanjang, W., Prachayasittikul, V., Prachayasittikul, S., & Phopin, K. (2021). Mechanisms and Neuroprotective Activities of Stigmasterol Against Oxidative Stress-Induced Neuronal Cell Death via Sirtuin Family. Frontiers in Nutrition, 8(May), 1–12. https://doi.org/10.3389/fnut.2021.648995
Saklayen, M. (2018). The epidemic of the metabolic syndrome. Current Hypertension Reports, 20(12), 1–8. https://doi.org/10.1007/s11906-018-0812-z
Sharma, K., Kumar, V., Kaur, J., Tanwar, B., Goyal, A., Sharma, R., Gat, Y., & Kumar, A. (2021). Health effects, sources, utilization and safety of tannins: a critical review. Toxin Reviews, 40(4), 432–444. https://doi.org/10.1080/15569543.2019.1662813
Ting, A. T., & Bertrand, M. J. M. (2016). More to Life than NF-κB in TNFR1 Signaling. Trends in Immunology, 37(8), 535–545. https://doi.org/10.1016/j.it.2016.06.002
Vergara-Jimenez, M., Almatrafi, M. M., & Fernandez, M. L. (2017). Bioactive components in Moringa oleifera leaves protect against chronic disease. Antioxidants, 6(4), 1–13. https://doi.org/10.3390/antiox6040091
Vyas, N. Y., & Raval, M. A. (2016). Aphrodisiac and spermatogenic potential of alkaloidal fraction of Hygrophila spinosa T. Ander in rats. Journal of Ethnopharmacology, 194, 947–953. https://doi.org/10.1016/j.jep.2016.10.080
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